The shotgun-based strategy—the technique of preference for sequencing large DNA genomes—has been used for the complete genome characterization of a range of eukaryotes and prokaryotes 1,2,3,4,5,6.
Sanger Sequencing, the workhorse of the human genome project, is still the gold standard technology for sequence analysis of plasmids and PCR products. The Genome Centre uses a third party to sequence ...